Mathieu featured from the start in Tuesday’s encounter, but was withdrawn in the 54th minute after sustaining the problem.
The Frenchman underwent a series of tests on Wednesday and Barcelona has now announced he will be unavailable for an estimated three weeks.
“Test conducted by the medical services on Wednesday morning have revealed that Jeremy Mathieu has injured the soleus in his right leg, which means he is going to be sidelined for an estimated three weeks,” a statement on the Catalans’ official website reads.
The 32-year-old has made six appearances in all competitions for Barcelona this term, scoring once in the process.
He received a red card in the 4-0 Champions League win over Manchester City last week.
Mathieu joins fellow defenders Gerard Pique and Jordi Alba on the sidelines, with the latter two suffering injuries against City.
